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Since a previous PIGU translocation was described as a germline event in a patient with bladder cancer Guo et al. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain. In total, this represents the preparation and processing of 69 lanes of Illumina Genome Analyzer II and the generation of more than 1 billion reads Table 1. In the outer ring, gray chromosome 20 and blue chromosome Indeed, the fact that we see no obvious change in PIGU expression in the fusion positive case when compared to the entire data set stands in contrast to the previous fusions that we identified. IKBKB levels are highest in the fusion positive sample black.

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Discovery of non-ETS gene fusions in human prostate cancer using next-generation RNA sequencing

This module also categorizes candidates and attributes various scores in order to sort and prioritize them for experimental follow-up. Article and publication date are at http: CitePeer Related Articles http: This may be because the snippet appears in a figure legend, contains special characters or spans different sections of rdrc2 article.

Footnotes [Supplemental material is available online at http: Nuclei were stained with DAPI blue. These types of targetable fusions may play a role in a personalized approach to cancer care. B RT-PCR and Sanger sequencing of the resulting fusion transcript reveals the expression of two different fusion transcripts.

Please fill out the form below and our representative will get back to you shortly. Nucleic Acids Res Long-range activation of FKBP51 transcription by the androgen receptor via distal intronic enhancers.

RSRC2 DepMap Gene Summary

Int J Oncol We used a novel computational approach, FusionSeq Sboner et al. However, these data suggest that, as exemplified by the plethora of ETV1 gene fusion partners Tomlins et al.

The gel dissolutions of all gel-based purification steps were conducted at room temperature under slight agitation as described by Quail et al. Despite the growing body of literature showing that IKK-beta exerts crucial function in inflammation, immunity, and cancer, the role of IKK-beta in human prostate cancer cells remains largely elusive.

Berger and colleagues demonstrate rare genomic rearrangements in human melanoma samples. Following retrieval, the sections were incubated sequentially with the primary antibody for 25 min, post-primary for 15 min, and polymer for 25 min ending with colorimetric development with diaminobenzidine DAB for 10 min Bond Polymer Refine Detection; Leica Microsystems.

Thus, this fusion likely impairs the proper function of these two genes. Protein lysates were prepared in the RIPA buffer radioimmunoprecipitation assay lysis buffer supplemented with protease inhibitor cocktail and phosphatase inhibitors Thermo Scientific. Clin Cancer Res Values from four wells were obtained for each treatment.

RSRC2 12q | bioCADDIE Data Discovery Index

Nor were there any evident differences in growth rates noted during standard passage of the subline compared to the parental cells with an estimated cell population doubling time of Summary of sequence data generation for 25 olw cancer samples PCa and three benign prostate tissue samples analyzed by next-generation whole transcriptome sequencing on Genome Analyzer II Illumina.

Following three washes with TBS-T, the blot was incubated with horseradish peroxidase—conjugated secondary antibody, and immune complexes were visualized by enhanced chemiluminescence detection ECL plus kit, GE Healthcare.

Within 72 h, cell rsfc2 in the BMStreated cells was significantly compromised compared to vehicle-treated cultures Fig. Although the number of samples is limited, this observation does raise the intriguing possibility that the presence of TMPRSS2—ERG rearrangements could be a prerequisite for subsequent oncogenic events. Curr Opin Genet Dev To begin with, we sought to validate selected read-through and cis candidate chimeras.

In the case of RNA extraction from benign tissue, we identified a frozen tissue block with no evidence of tumor tissue to minimize the possibility of tumor contamination in the benign tissue sample. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

Open in a separate window. In the outer ring, gray chromosome 20 and blue chromosome The total protein concentration of the soluble extract was determined using the BCA protein assay Kit Thermo Scientific. Acknowledgments We thank S.